Description
Aviva Systems Biology GHRH ELISA Kit (Human) (OKEH00447) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human GHRH antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human GHRH and incubated. The Human GHRH found in the sample or standards competes with the biotinylated Human GHRH for limited binding sites on the immobilized anti-Human GHRH antibody. Excess unbound biotinylated Human GHRH and sample or standard Human GHRH is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human GHRH captured in the well and inversely proportional to the amount of Human GHRH which was contained in the sample or standard